Goat Anti-CD74 (aa159-171) Antibody

$423.00

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SKU: EB12551
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
gamma chain of class II antigens| MHC HLA-DR gamma chain| Ia-associated invariant chain| HLA-DR-gamma| CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated)| CD74 antigen| CD74 molecule, major histocompatibility complex, class II invariant chain| Ia-GAMMA| HLADG| DHLAG|CD74
Usage Summary

Flow Cytometry: Flow cytometric analysis of Caco--2 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_001020330.1; NP_004346.1; NP_001351012.1; : NP_001351013.1
Blocking Peptide
EBP12551
Immunogen
Peptide with sequence C-KGSFPENLRHLKN, from the internal region of the protein sequence according to NP_001020330.1; NP_004346.1; NP_001351012.1; : NP_001351013.1.
Product Comments
This antibody is expected to recognize all reported isoforms.
Peptide Sequence
C-KGSFPENLRHLKN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
972
Product Grade
IHC Results
Paraffin embedded Human Spleen. Recommended concentration: 5µg/ml
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
This antibody was observed to recognize variations of bands at approx. 26kDa, 30kDa,35kDa and 70kDa in Human Duodenum and Lung lysates (calculated MW of 26.4kDa according to NP_004346.1 and 33.5kDa according to NP_001020330.1. All bands were blocked by incubation with the immunizing peptide and have been observed by other commercial sources. Recommended concentration: 0.5-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, FC