Goat Anti-CHCHD3 Antibody

$423.00

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SKU: EB09839
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
coiled-coil-helix-coiled-coil-helix domain containing 3|FLJ20420|CHCHD3
Accession ID
NP_060282.1
Blocking Peptide
EBP09839
Immunogen
Peptide with sequence KFKRYESHPVCAD, from the internal region of the protein sequence according to NP_060282.1.
Peptide Sequence
KFKRYESHPVCAD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow, Sheep
Reactive Species
Human
Human Gene ID
54927
Rat Gene ID
296966
Product Grade
IHC Results
Paraffin embedded Human Heart and Skeletal Muscle. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 26kDa band observed in Human Brain (Cerebellum) and Heart lysates (calculated MW of 26.2kDa according to NP_060282.1). Recommended concentration: 1-3µg/ml. A customer found ~30kDa in THP1 and in Human primary monocyte derived dendritic cells.
Application Type
Pep-ELISA, WB, IHC
Selected References
[{"pmid": 28057766, "intro": "This antibody has been successfully used in IF on Human:", "title": "Rapid degradation of mutant SLC25A46 by the ubiquitin-proteasome system results in MFN1/2 mediated hyperfusion of mitochondria.", "author": "Steffen J, Vashisht AA, Wan J, Jen JC, Claypool SM, Wohlschlegel J, Koehler CM.", "journal": "Mol Biol Cell. 2017 Jan 5."}]