Goat Anti-CRP2 / CSRP2 Antibody

$423.00

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SKU: EB08182
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
LIM domain only 5, smooth muscle|SmLIM|LMO5|cysteine and glycine-rich protein 2|CSRP2|CRP2
Accession ID
NP_001312.1
Blocking Peptide
EBP08182
Immunogen
Peptide with sequence C-ERLGIKPESVQP, from the internal region of the protein sequence according to NP_001312.1.
Peptide Sequence
C-ERLGIKPESVQP
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Horse
Reactive Species
Human, Mouse, Rat
Human Gene ID
1466
Mouse Gene ID
13008
Product Grade
IHC Results
In paraffin embedded Human Lung shows staining of the endothelial cells of a blood vessel. Recommended concentration: 3-6µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx 26kDa band observed in Human, Mouse and Rat Kidney lysates (calculated MW of 21.0kDa according to NP_001312.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Huber et al, J Biol Chem. 2000 Feb 25;275(8):5504-11; PMID: 10681529). In transfected HEK293 transiently expressing CRP2 a band of approx. 26kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC