Goat Anti-CTDSPL Antibody

$423.00

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SKU: EB08525
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
small CTD phosphatase 3|SCP3|PSR1|HYA22|C3orf8|CTD (carboxy-terminal domain, RNA polymerase II, polypeptide A) small phosphatase-like|CTDSPL
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei and vesicles of A431 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_001008393.1; NP_005799.2
Blocking Peptide
EBP08525
Immunogen
Peptide with sequence QCNVSLKKQRSRS, from the internal region of the protein sequence according to NP_001008393.1; NP_005799.2.
Product Comments
This antibody is expected to recognise both isoforms (NP_001008393.1; NP_005799.2).
Peptide Sequence
QCNVSLKKQRSRS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Dog
Reactive Species
Human
Human Gene ID
10217
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary testing showed a band at approx 28kDa in nuclear lysate of cell lines A431 and HeLa, after 1-2µg/ml antibody staining (calculated Mwt of 29.9kDa according to NP_005799.2). Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IF, FC