Goat Anti-DDAH1 Antibody

$464.00

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SKU: EB05278
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
FLJ25539|FLJ21264|RP4-621F18.1|NG, NG-dimethylarginine dimethylaminohydrolase|DDAHI|DDAH|dimethylarginine dimethylaminohydrolase 1|DDAH1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in HeLa and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_036269.1; NP_001127917.1; NP_001317584.1
Blocking Peptide
EBP05278
Immunogen
Peptide with sequence TCCSVLINKKVDS, from the C Terminus of the protein sequence according to NP_036269.1; NP_001127917.1; NP_001317584.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_036269.1and NP_001127917.1).
Peptide Sequence
TCCSVLINKKVDS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Cow
Reactive Species
Human
Human Gene ID
23576
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:1000.
Western Blot
Approx 37kDa band observed in Human Cerebellum, Kidney and Testis lysates, and in lysates of cell lines HepG2 and LNCaP (calculated MW of 31.1kDa according to NP_036269.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC