Goat Anti-DEFA1/3 precursor Antibody

$423.00

Category: Primary Antibodies
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SKU: EB09742
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
neutrophil peptide 3|defensin, alpha 3, neutrophil-specific|DEFA3|myeloid-related sequence|defensin, alpha 1|MRS|HP-1|HNP-1|DEF1|HGNC:2761|defensin, alpha 1, myeloid-related sequence|DEFA1
Accession ID
NP_004075.1; NP_005208.1
Blocking Peptide
EBP09742
Immunogen
Peptide with sequence C-DESLAPKHPGSRKN, from the internal region of the protein sequence according to NP_004075.1; NP_005208.1.
Product Comments
This antibody is expected to equally recognize DEFA1 precursor and DEFA3 precursor.
Peptide Sequence
C-DESLAPKHPGSRKN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
1667, 1668
Product Grade
IHC Results
Paraffin embedded Human Thymus. Recommended concentration: 3-6µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 10kDa band observed in Human Spleen lysates (calculated MW of 10.2kDa according to NP_004075.1). An additional band of unknown identity was also consistently observed at 20kDa, and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC

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