Goat Anti-DLG1 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB08001
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
synapse-associated protein 97|presynaptic protein SAP97|discs, large homolog 1|discs large homolog 1|hdlg|dJ1061C18.1.1|SAP97|DLGH1|DKFZp781B0426|DKFZp761P0818|discs, large homolog 1 (Drosophila)|DLG1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm and ER of HeLa and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_001091894.1; NP_004078.2; NP_001191316.1; NP_001191317.1; NP_001353132.1; NP_001353133.1; NP_001353140.1; NP_001353145.1; NP_001353150.1; NP_001353151.1
Blocking Peptide
EBP08001
Immunogen
Peptide with sequence C-NSKTRDKGEIPDD, from the internal region of the protein sequence according to NP_001091894.1; NP_004078.2; NP_001191316.1; NP_001191317.1; NP_001353132.1; NP_001353133.1; NP_001353140.1; NP_001353145.1; NP_001353150.1; NP_001353151.1.
Peptide Sequence
C-NSKTRDKGEIPDD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat
Reactive Species
Human
Human Gene ID
1739
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Preliminary testing showed a band at approx 150kDa in lysate of cell line Kelly after 0.3µg/ml antibody staining (calculated MW of 103kDa according to NP_004078.2). This molecular weight is observed by other sources. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IF, FC

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