Goat Anti-EAAT1 / GLAST1 (aa197-208) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

SLC1A3|solute carrier family 1 (glial high affinity glutamate transporter), member 3|EA6|EAAT1|FLJ25094|GLAST|GLAST1|GLAST-1|OTTHUMP00000116002|excitatory amino acid transporter 1|sodium-dependent glutamate/aspartate transporter 1|solute carrier family 1 member 3

NP_004163.3; NP_001160167.1

EBP11915

Peptide with sequence C-RSFKVPIQANET, from the internal region of the protein sequence according to NP_004163.3; NP_001160167.1.

This antibody is expected to recognize isoform 1 (NP_004163.3) and isoform 2 (NP_001160167.1).

C-RSFKVPIQANET

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Rat

Human

6507

29483

Antibody detection limit dilution 1:128000.

Approx 60kDa band observed in Human Brain (Frontal Cortex) lysates (calculated MW of 59.6kDa according to NP_004163.3). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 22kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA, WB