Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names OTTHUMP00000207060|OTTHUMP00000207056|MGC126406|ELMO-1|CED-12|engulfment and cell motility 1|ced-12 homolog 1|KIAA0281|CED12|engulfment and cell motility 1 (ced-12 homolog, C. elegans)|ELMO1 | |
Usage Summary Immunoprecipitation: A customer found positive results in IP on Human promyelocytic leukemia cells (HL60). This data is from a previous batch, not on sale. | |
Accession ID NP_055615.8; NP_569709.1; NP_001034548.1 | |
Blocking Peptide EBP05297 | |
Immunogen Peptide with sequence PKEPSNYDFVYDCN, from the C Terminus of the protein sequence according to NP_055615.8; NP_569709.1; NP_001034548.1. | |
Product Comments This antibody is expected to recognise both human isoforms of this protein. NP_569709.1 and NP_001034548.1 represent the same isoform. | |
Peptide Sequence PKEPSNYDFVYDCN | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Cow, Zebrafish | |
Reactive Species Human, Mouse, Rat | |
Human Gene ID 9844 | |
Mouse Gene ID 140580 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Approx 83kDa band observed in Human Brain (Frontal Cortex) lysates, in Mouse and Rat Brain lysates and in lysates of cell line Jurkat (calculated MW of 83.8kDa according to NP_055615.8). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. | |
Application Type Pep-ELISA, WB |
Goat Anti-ELMO1 Antibody
$423.00
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Selected References [{"pmid": 25586182, "intro": "This antibody (previous batch) has been successfully used in Western blot in Human:", "title": "The bipartite rac1 Guanine nucleotide exchange factor engulfment and cell motility 1/dedicator of cytokinesis 180 (elmo1/dock180) protects endothelial cells from apoptosis in blood vessel development.", "author": "Schäker K, Bartsch S, Patry C, Stoll SJ, Hillebrands JL, Wieland T, Kroll J.", "journal": "J Biol Chem. 2015 Mar 6;290(10):6408"}, {"pmid": 27849017, "intro": "This antibody (previous batch) has been successfully used in Western blot in Zebrafish:", "title": "ELMO1 protects renal structure and ultrafiltration in kidney development and under diabetic conditions.", "author": "Krishna Rakesh Sharma, Karl Heckler, Sandra J. Stoll, Jan-Luuk Hillebrands, Katharina Kynast, Esther Herpel, Stefan Porubsky, Marlies Elger, Boris Hadaschik, Karen Bieback, Hans-Peter Hammes, Peter P. Nawroth, and Jens Kroll", "journal": "Sci Rep. 2016 Nov 16;6:37172"}, {"pmid": 20466982, "intro": "This antibody (previous batch) has been successfully used in Western blot and IP in Zebrafish:", "title": "The Rac1 regulator ELMO1 controls vascular morphogenesis in zebrafish.", "author": "Epting D, Wendik B, Bennewitz K, Dietz CT, Driever W, Kroll J.", "journal": "Circ Res. 2010 Jul 9;107(1):45-55."}] |