Goat Anti-ELMO2 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB05305
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
ELMO-2|CED-12|RP11-394O2.2|engulfment and cell motility 2|engulfment and cell motility 2|PH domain protein CED12A|ced-12 homolog 2|KIAA1834|FLJ11656|CED12|engulfment and cell motility 2 (ced-12 homolog, C. elegans)|ELMO2
Usage Summary
Immunofluorescence: Strong expression of the protein seen in A549 and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_877496.1; NP_573403.1; NP_001305182.1
Blocking Peptide
EBP05305
Immunogen
Peptide with sequence C-PKEPSSYDFVYHYG, from the C Terminus of the protein sequence according to NP_877496.1; NP_573403.1; NP_001305182.1.
Peptide Sequence
C-PKEPSSYDFVYHYG
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human, Mouse, Rat
Human Gene ID
63916
Mouse Gene ID
140579
Product Grade
IHC Results
In paraffin embedded Human Prostate shows pixulated staining in the cytoplasm of stroma cells. Recommended concentration: 3-6µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 80kDa band observed in lysates of cell lines HEK293, Jurkat, RAW264.7 and in Human Cerebellum and Rat Brain lysates (calculated MW of 82.6kDa according to Human NP_573403.1 and NP_877496.1 and Mouse NP_525026.2, and 83.7kDa according tp Rat NP_001128427.1). Recommended concentration: 0.3-1ug//ml.
Application Type
Pep-ELISA, WB, IHC, IF, FC

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