Goat Anti-ELMO3 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB05295
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
ELMO-3|CED-12|engulfment and cell motility 3|ced-12 homolog 3|FLJ13824|CED12|engulfment and cell motility 3 (ced-12 homolog, C. elegans)|ELMO3
Usage Summary
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.
Accession ID
NP_078988.3
Blocking Peptide
EBP05295
Immunogen
Peptide with sequence TNFNFCYDCSIAEP, from the C Terminus of the protein sequence according to NP_078988.3.
Peptide Sequence
TNFNFCYDCSIAEP
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
79767
Product Grade
IHC Results
In paraffin embedded Human Prostate shows vesiculate staining in the cytoplasm of secretory cells. Recommended concentration, 5-10µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx. 75kDa band observed in lysates of cell line A431 and in Human Tonsil lysates (calculated MW of 70.0kDa according to NP_ 078988.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, FC

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