Goat Anti-EPB41L2 / 4.1G (aa 593 to 604) Antibody

$464.00

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SKU: EB08135
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
erythrocyte membrane protein band 4.1 like-protein 2|OTTHUMP00000040262|DKFZp781H1755|DKFZp781D1972|RP3-324N14.1|erythrocyte membrane protein band 4.1-like 2|4.1-G|EPB41L2
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the membranes of A431 and in the cell junctions of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_001422.1; NP_001129026.1; NP_001186317.1; NP_001239589.1; NP_001337228.1; NP_001337230.1; NP_001337232.1; NP_001337233.1; NP_001337236.1; NP_001337243.1; NP_001337249.1
Blocking Peptide
EBP08135
Immunogen
Peptide with sequence C-RREVRSPTKAPH, from the internal region of the protein sequence according to NP_001422.1; NP_001129026.1; NP_001186317.1; NP_001239589.1; NP_001337228.1; NP_001337230.1; NP_001337232.1; NP_001337233.1; NP_001337236.1; NP_001337243.1; NP_001337249.1.
Product Comments
This antibody is reported to recognise all isoforms NP_001422.1 (iso a), NP_001129026.1 (iso b), NP_001186317.1 (iso c), NP_001239589.1 (iso d), NP_001337228.1 (iso e), NP_001337230.1 (iso f), NP_001337232.1 (iso g),NP_001337233.1 (iso h), NP_001337236.1 (iso i), NP_001337243.1 (iso j), NP_001337249.1 (iso k).
Peptide Sequence
C-RREVRSPTKAPH
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
2037
Product Grade
IHC Results
Paraffin embedded Human Uterus and Colon. Recommended concentration: 2.5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary testing showed bands at approx 110+40kDa in Human Cerebellum lysatesafter 0.1µg/ml antibody staining (calculated MW of 113kDa according to NP_001422.1 These bands were successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IF, IHC, FC