Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names ephrin receptor EphB6|MGC129911|MGC129910|HEP|EPH receptor B6|EPHB6 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the membranes and cytoplasm of HeLa and U2OS cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_004436.2; NP_001267723.2 | |
Blocking Peptide EBP09557 | |
Immunogen Peptide with sequence C-EVIAHGKHTTSSD, from the internal region of the protein sequence according to NP_004436.2; NP_001267723.2. | |
Peptide Sequence C-EVIAHGKHTTSSD | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog | |
Reactive Species Human | |
Human Gene ID 2051 | |
Mouse Gene ID 13848 | |
Rat Gene ID 312275 | |
Product Grade  | |
IHC Results Paraffin embedded Human Small Intestine and Brain (Cerebellum). Recommended concentration: 3.75µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Preliminary testing showed a band at approx 80kDa in U251 cell lysate and approx.75kDa in Human Pancreas lysate after 1-2µg/ml antibody staining (calculated MW of 79.8kDa according to NP_001267723.2). Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, IF, IHC, FC |
Goat Anti-EPHB6 Antibody
$464.00
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