Goat Anti-FOXG1 / BF2 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB05608
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
oncogene QIN|forkhead-like 4|forkhead-like 3|forkhead-like 2|forkhead-like 1|forkhead, drosophila, homolog-like 2|forkhead box G1C|forkhead box G1B|forkhead box G1A|brain factor 2|OTTHUMP00000178764|QIN|KHL2|HFK3|HFK2|HFK1|HBF2|HBF-G2|HBF-3|HBF-2|HBF-1|FOXG1C|FOXG1B|FOXG1A|FKHL4|FKHL3|FKHL2|FKHL1|FKH2|FHKL3|BF2|BF1|forkhead box G1
Usage Summary
Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended concentration: 10ug/ml.
Accession ID
NP_005240.3
Blocking Peptide
EBP05608
Immunogen
Peptide with sequence C-HQNQGSSSNPLIH, from the C Terminus of the protein sequence according to NP_005240.3.
Peptide Sequence
C-HQNQGSSSNPLIH
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
2290
Mouse Gene ID
15228
Rat Gene ID
24370
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx. 48kDa band observed in lysates of cell line U251, and approx. 48-50kDa in Human Cerebellum and Cerebral Cortex lysates (calculated MW of 52.4kDa according to NP_005240.3 (FOXG1B), and 50.5kDa according to CAA55038.1 (FOXG1A). An additional band at 70-75kDa was observed in some lysates and was successfullu blocked by incubation with the immunizing peptide, Recommended concentration: 0.3-1/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, FC

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