Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names forkhead box K2|cellular transcription factor ILF-1|interleukin enhancer binding factor 1|ILF-1|ILF|FOXK2|ILF1 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasmic/vesicles of U2OS and HeLa cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_004505.2; | |
Blocking Peptide EBP06034 | |
Immunogen Peptide with sequence C-TPPAAVREKGVQN, from the C Terminus of the protein sequence according to NP_004505.2;. | |
Peptide Sequence C-TPPAAVREKGVQN | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human | |
Human Gene ID 3607 | |
Product Grade ![]() | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Approx 80kDa band observed in nuclear lysates of cell lines HEK293, HeLa and Jurkat (calculated MW of 69.1kDa according to NP_004505.2.) This molecular weight is routinely observed by other sources and was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.01-0.03µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IF, FC |
Goat Anti-FOXK2 / ILF (isoform 1) Antibody
$464.00
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