Goat Anti-FXR1 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

fragile X mental retardation-related protein 1|fragile X mental retardation, autosomal homolog 1|FXR1

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

NP_005078.2; NP_001013456.1; NP_001013457.1

EBP07361

Peptide with sequence C-RIEGDNENKLPRED, from the internal region of the protein sequence according to NP_005078.2; NP_001013456.1; NP_001013457.1.

This antibody is expected to recognise all reported isoforms (NP_005078.2, NP_001013456.1 and NP_001013457.1).

C-RIEGDNENKLPRED

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Cow

Human, Mouse

8087

14359

361927

Antibody detection limit dilution 1:128000.

Approx 75+80kDa band observed in lysates of cell line NIH-3T3 (calculated MW of 59.9kDa according to NP_001013457.1). The observed molecular weight corresponds to earlier findings in literature (Khandjian et al Hum Mol Genet. 1998 Dec;7(13):2121-8.; PMID: 9817930). An additional band of unknown identity was also consistently observed at 37kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration. 1-3µg/ml. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, IF, FC