Goat Anti-GABPB2 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

nuclear respiratory factor-2 subunit beta|GA binding protein transcription factor, beta subunit 2, 47kDa|GA binding protein transcription factor, beta subunit 1, 53kDa|NRF2B2|NRF2B1|GABPB1|GABPB|E4TF1B|E4TF1-53|E4TF1-47|E4TF1|BABPB2|GA binding protein transcription factor, beta subunit 2|GABPB2

Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

NP_005245.2; NP_002032.2; NP_001307844.1

EBP07818

Peptide with sequence C-SSENSSKATDETG, from the internal region of the protein sequence according to NP_005245.2; NP_002032.2; NP_001307844.1.

C-SSENSSKATDETG

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Dog

Human

2553

Paraffin embedded Human Tonsil and Spleen. Recommended concentration: 3.75µg/ml.

Antibody detection limit dilution 1:32000.

Approx 45+38kDa bands observed in lysates of cell line HeLa (calculated MW of 42.5kDa according to NP_005245.2, NP_001307844.1 and of 38.1kDa according to NP_002032.2). An additional band of unknown identity was also consistently observed at 22kDa and was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, IHC, IF, FC