Goat Anti-GAD1 (isoform GAD67) Antibody

$464.00

Category: Primary Antibodies
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SKU: EB09109
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
GAD25|GAD67|glutamate decarboxylase 1 (brain, 67kD)|glutamate decarboxylase 1|OTTHUMP00000041055|SCP|GAD|FLJ45882|glutamate decarboxylase 1 (brain, 67kDa)|GAD1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the membrane and cytoplasm of A431 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_000808.2
Blocking Peptide
EBP09109
Immunogen
Peptide with sequence C-PDSPQRREKLHK, from the internal region of the protein sequence according to NP_000808.2.
Product Comments
This antibody is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.
Peptide Sequence
C-PDSPQRREKLHK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow, Pig
Reactive Species
Human, Mouse, Rat
Human Gene ID
2571
Mouse Gene ID
14415
Rat Gene ID
24379
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 70kDa band observed in Human Cerebellum, and in Mouse and Rat Brain lysates (calculated MW of 66.9kDa according to Human NP_000808.2, and 66.6kDa according to Mouse NP_032103.2 and Rat NP_058703.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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