Goat Anti-GIPC1 / NIP Antibody

$423.00

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SKU: EB06196
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
SYNECTIN|synectin|regulator of G-protein signalling 19 interacting protein 1|IGF-1 receptor interacting protein 1|GLUT1 C-terminal binding protein|TIP-2|SYNECTIIN|SEMCAP|RGS19IP1|MGC3774|MGC15889|IIP-1|Hs.6454|GLUT1CBP|GIPC|C19orf3|GIPC PDZ domain containing family, member 1|NIP|GIPC1
Accession ID
NP_005707.1; NP_974197.1; NP_974199.1; NP_974196.1; NP_974198.1; NP_974223.1
Blocking Peptide
EBP06196
Immunogen
Peptide with sequence C-GAIGDAKVGRY, from the C Terminus of the protein sequence according to NP_005707.1; NP_974197.1; NP_974199.1; NP_974196.1; NP_974198.1; NP_974223.1.
Product Comments
This antibody is expected to recognize both isoform 1 (NP_005707.1, NP_974197.1 and NP_974199.1) and isoform 2 (NP_974196.1, NP_974198.1 and NP_974223.1).
Peptide Sequence
C-GAIGDAKVGRY
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Cow
Reactive Species
Human
Human Gene ID
10755
Mouse Gene ID
67903
Rat Gene ID
83823
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 38kDa and strong 23-24kDa bands observed in lysates of cell line HeLa, and approx 24kDa also observed in Human Pancreas, Kidney and Muscle lysates (calculated MW of 36.0kDa according to NP_005707.1, NP_974197.1 and NP_974199.1 and 26.1kDa according to NP_974198.1 and NP_974223.1). In transfected HEK293 transiently expressing GIPC1 isoform 1 a band of approx. 40kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.03-0.1µg/ml.
Application Type
Pep-ELISA, WB