Goat Anti-GNAS Antibody

$423.00

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SKU: EB10063
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
GNAS|POH|PHP1B|PHP1A|OTTHUMP00000196030|OTTHUMP00000196026|OTTHUMP00000031742|NESP|MGC33735|GSP|GSA|GPSA|GNAS1|GNAS complex locus|dJ309F20.1.1|C20orf45|AHO|adenylate cyclase-stimulating G alpha protein
Accession ID
NP_000507.1; NP_001070956.1; NP_001070957.1
Blocking Peptide
EBP10063
Immunogen
Peptide with sequence C-QAARSNSDGEKATK, from the internal region of the protein sequence according to NP_000507.1; NP_001070956.1; NP_001070957.1.
Product Comments
This antibody is expected to recognize all three reported isoforms NP_000507.1, NP_001070956.1 and NP_536350.2. However, in Mouse it is expected to recognize reporterd isoforms GNASL (NP_963910.1) and XLas (NP_034439.2) only.
Peptide Sequence
C-QAARSNSDGEKATK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Cow
Reactive Species
Human, Mouse, Rat
Human Gene ID
2778
Mouse Gene ID
14683
Rat Gene ID
24896
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx. 45kDa band observed in Human Cerebellum, Mouse and Rat Brain lysates, and in lysates of cell line KNRK (calculated MW of 45.6kDa according to Human NP_963910.1, Mouse NP_963910.1 and Rat NP_062005.1). Recommended concentration: 0.1-0.5µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB
Selected References
[{"pmid": 30377371, "intro": "This antibody (previous batch) has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}]