Goat Anti-HMGA1 (aa9-21) Antibody

$423.00

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SKU: EB09567
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y|high-mobility group (nonhistone chromosomal) protein isoforms I and Y|OTTHUMP00000039618|OTTHUMP00000016224|OTTHUMP00000016223|OTTHUMP00000016222|MGC4854|MGC4242|MGC12816|HMGIY|HMGA1A|HMG-R|high mobility group AT-hook 1|HMGA1
Accession ID
NP_665906.1; NP_002122.1
Blocking Peptide
EBP09567
Immunogen
Peptide with sequence C-SQPLASKQEKDGT, from the internal region (near N Terminus) of the protein sequence according to NP_665906.1; NP_002122.1.
Product Comments
This antibody is expected to recognize reported isoforms a (NP_665906.1, NP_665908.1, NP_665911.1) and b (NP_002122.1, NP_665910.1, NP_665912.1). This antibody is not expected to cross-react with HMGA2.
Peptide Sequence
C-SQPLASKQEKDGT
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow, Pig
Reactive Species
Human
Human Gene ID
3159
Mouse Gene ID
15361
Rat Gene ID
117062
Product Grade
IHC Results
Paraffin embedded Human Skin and Placenta. Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 24kDa band observed in Human Pancreas (calculated MW of 11.7kDa/10.7kDa according to NP_665906.1; NP_002122.1). This molecular weight is routinely observed by other sources. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 0.5-1µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC