Goat Anti-HP alpha chain Antibody

$423.00

Category: Primary Antibodies
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SKU: EB11197
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
HP| MGC111141| HPA1S| HP2ALPHA2| haptoglobin, beta polypeptide| haptoglobin, alpha polypeptide| haptoglobin alpha(2FS)-beta| haptoglobin alpha(1S)-beta| haptoglobin| BP| binding peptide
Usage Summary
Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.
Accession ID
NP_005134.1; NP_001119574.1; NP_001305067.1
Blocking Peptide
EBP11197
Immunogen
Peptide with sequence QCKNYYKLRTEGD, from the internal region of the protein sequence according to NP_005134.1; NP_001119574.1; NP_001305067.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_005134.1; NP_001119574.1).
Peptide Sequence
QCKNYYKLRTEGD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
3240
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 18kDa band observed in Human Liver lysates and approx. 20kDa in preliminary testing of HepG2 cell lysate (calculated MW of 18kDa according to alpha chain cleaved from NP_005134.1). Recommended concentration: 0.01-0.03µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, FC

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