Goat Anti-HYPE / FICD Antibody

$464.00

Category: Primary Antibodies
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SKU: EB10860
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
huntingtin interacting protein 13| fic S-phase protein cell division homolog| Huntingtin interacting protein E| UNQ3041| MGC5623| HYPE| HIP13| FIC domain containing|FICD
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_009007.2
Blocking Peptide
EBP10860
Immunogen
Peptide with sequence C-PPITIRKEQRSD, from the internal region of the protein sequence according to NP_009007.2.
Peptide Sequence
C-PPITIRKEQRSD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
11153
Mouse Gene ID
231630
Rat Gene ID
288741
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx. 55kDa band observed in Human Tonsil and Kidney lysates, approx. 55-60kDa in lysates of cell lines HeLa and HEK293, and approx. 60kDa in HepG2 cell lysate, which was successfully blocked by incubation with the immunizing peptide (calculated MW of 51.8kDa according to NP_009007.2). Recommended concentration: 0.1-0.5µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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