Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
| |
Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names intraflagellar transport 88 homolog (Chlamydomonas)|recessive polycystic kidney disease protein Tg737 homolog|intraflagellar transport 88 homolog|Tg737 protein|Probe hTg737 (polycystic kidney disease, autosomal recessive)|TPR repeat protein 10|hTg737|TG737|RP11-172H24.2|MGC26259|IFT88|DAF19|D13S1056E|HGNC:20606|tetratricopeptide repeat domain 10|TTC10|polaris|IFT88 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A549 and HepG2 cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of THP-1 cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_783195.2; NP_006522.2, NP_001340507.1; NP_001340498.1 | |
Blocking Peptide EBP07088 | |
Immunogen Peptide with sequence C-KKRIDEDDFADEE, from the C Terminus of the protein sequence according to NP_783195.2; NP_006522.2, NP_001340507.1; NP_001340498.1. | |
Product Comments This antibody is expected to recognise many reported isoforms. | |
Peptide Sequence C-KKRIDEDDFADEE | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog | |
Reactive Species Human, Rat | |
Human Gene ID 8100 | |
Mouse Gene ID 21821 | |
Rat Gene ID 305918 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:8000. | |
Western Blot Approx. 80kDa band observed in lysates of cell lines THP-1, K562 and KNRK and 85kDa in lysates of cell line U251 (calculated MW of 94.3kDa according to Human NP_783195.2) and 93.1kDa according to Rat XP_006252128.1) . These bands were successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
A batch specific positive control lysate is available for this product. Please contact [email protected] for availability. | |
Application Type Pep-ELISA, WB, IF, FC |
Goat Anti-IFT88 / Polaris Antibody
$423.00
| SKU | Unit Size | Price |
|---|---|---|
Select a unit size:
Selected References [{"pmid": 19470799, "intro": "This antibody (previous batch) has been successfully used in IF on Mouse:", "title": "Pericentrin, a centrosomal protein related to microcephalic primordial dwarfism, is required for olfactory cilia assembly in mice.", "author": "Miyoshi K, Kasahara K, Miyazaki I, Shimizu S, Taniguchi M, Matsuzaki S, Tohyama M, Asanuma M.", "journal": "FASEB J. 2009 Oct;23(10):3289-97."}] |