Goat Anti-IGF2BP1/IMP1 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

IGF2BP1|zipcode-binding protein 1|ZBP1|VICKZ1|VICKZ family member 1|insulin-like growth factor 2 mRNA binding protein 1|IMP-1|IMP1|IGF2 mRNA-binding protein 1|IGF II mRNA binding protein 1|CRD-BP|CRDBP|coding region determinant-binding protein

Immunofluorescence: Strong expression of the protein seen in HepG2 and NIH3T3 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

NP_006537.3; NP_001153895.1

EBP10067

Peptide with sequence C-EKVFAEHKISYSGQ, from the internal region of the protein sequence according to NP_006537.3; NP_001153895.1.

This antibody is expected to recognize both reported isoforms (NP_006537.3; NP_001153895.1).

C-EKVFAEHKISYSGQ

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Cow

Human, Mouse

10642

140486

303477

Paraffin embedded Human Breast. Recommended concentration: 3.75µg/ml.

Antibody detection limit dilution 1:16000.

Approx 65kDa band observed in lysates of cell line Caco-2 and approx. 70kDa in lysates of cell line K562 and in nuclear cell lysates of HepG2 and NIH3T3 (calculated MW of 63.5kDa according to Human NP_006537.3 and Mouse NP_034081.1). Recommended concentration: 0.1-1µg/ml. Primary incubation 1 hour at room temperature. Positive Control: A batch specific positive control lysate is available for this product. Please contact [email protected] for availability.

Pep-ELISA, WB, IHC, IF, FC