Goat Anti-IKAROS / IKZF1 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB09708
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
lymphoid transcription factor LyF-1|zinc finger protein, subfamily 1A, 1 (Ikaros)|Ikaros (zinc finger protein)|CLL-associated antigen KW-6|hIk-1|ZNFN1A1|PRO0758|LYF1|IKAROS|IK1|Hs.54452|IKAROS family zinc finger 1 (Ikaros)|IKZF1
Accession ID
NP_006051.1; NP_001278766.1;NP_001278767.1;NP_001207696.1;NP_001207697.1; NP_001278768.1; NP_001207699.1; NP_001207700.1
Blocking Peptide
EBP09708
Immunogen
Peptide with sequence C-DTPYDSSASYEKEN, from the internal region of the protein sequence according to NP_006051.1; NP_001278766.1;NP_001278767.1;NP_001207696.1;NP_001207697.1; NP_001278768.1; NP_001207699.1; NP_001207700.1.
Product Comments
This antibody is expected to recognise all isoforms NP_001278766.1;NP_001278767.1;NP_001207696.1;NP_001207697.1; NP_001278768.1; NP_001207699.1; NP_001207700.1
Peptide Sequence
C-DTPYDSSASYEKEN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Dog, Cow
Reactive Species
Human
Human Gene ID
10320
Product Grade
IHC Results
In paraffin embedded Human Tonsil shows textured staining in some nuclei of the lymphoid tissue. Recommended concentration: 2.5-3.8µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:1000.
Western Blot
Preliminary testing showed a band at approx. 60kDa in Human Lymph node and Peripheral Blood Monocyte lysate, after 1ug/ml antibody staining (calculated MW of 57.5kDa according to NP_006051.1). Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IHC

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