Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names RP23-415G1.3|pp160|phosphoprotein of 160 kDa|IRS-4|Irs4|insulin receptor substrate 4 | |
Usage Summary Immunofluorescence: This product has been successfully used in IF on Mouse: Harriet M Jackson et al, BMC Genomics. 2013 Nov 25;14:831. PMID: 24274089. | |
Accession ID NP_034702.2 | |
Blocking Peptide EBP11828 | |
Immunogen Peptide with sequence C-DRGTKERKEAKEVRD, from the internal region of the protein sequence according to NP_034702.2. | |
Peptide Sequence C-DRGTKERKEAKEVRD | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Mouse, Rat | |
Reactive Species Mouse | |
Mouse Gene ID 16370 | |
Rat Gene ID 315350 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Approx 150kDa band observed in Mouse Heart lysates (calculated MW of 130kDa according to NP_034702.2). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Schreyer et al, jEndocrinology. 2003 Apr;144(4):1211-8. PMID: 12639902). Recommended concentration: 0.2-0.6µg/ml. Primary incubation was 1 hour. An additional band of unknown identity was also consistently observed at ~39kDa. This band was successfully blocked by incubation with the immunizing peptide. This product has been successfully used in WB on Mouse: Ikink GJ et al, Nat Commun. 2016 Nov 23;7:13567. PMID: 27876799. | |
Application Type Pep-ELISA, WB, IF |
Goat Anti-Irs4 (mouse aa633-647) Antibody
$423.00
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Selected References [{"pmid": 24274089, "intro": "This antibody has been successfully used in IF on Mouse:", "title": "Clustering of transcriptional profiles identifies changes to insulin signaling as an early event in a mouse model of Alzheimer’s disease", "author": "Harriet M Jackson, Ileana Soto, Leah C Graham, Gregory W Carter and Gareth R Howel.", "journal": "BMC Genomics. 2013 Nov 25;14:831. "}, {"pmid": 27876799, "intro": "This antibody has been successfully used in Western blot on Mouse:", "title": "IRS4 induces mammary tumorigenesis and confers resistance to HER2-targeted therapy through constitutive PI3K/AKT-pathway hyperactivation", "author": "Ikink GJ, Boer M, Bakker ER, Hilkens J", "journal": "Nat Commun. 2016 Nov 23;7:13567"}] |