Goat Anti-KIT / CD117 Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

soluble KIT varian| proto-oncogene tyrosine-protein kinase Kit| proto-oncogene c-Kit| piebald trait protein| p145 c-kit| mast/stem cell growth factor receptor Kit| SCFR| PBT| CD117| C-Kit| v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog|KIT

Immunofluorescence: Strong expression of the protein seen in the cytoplasm/ membranes of HEK293 and HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.

NP_000213.1; NP_001087241.1

EBP12145

Peptide with sequence KRDSFICSKQEDH, from the internal region of the protein sequence according to NP_000213.1; NP_001087241.1.

This antibody is expected to recognize both reported isoforms (NP_000213.1; NP_001087241.1).

KRDSFICSKQEDH

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Dog, Pig, Cow

Human

3815

Paraffin embedded Human Prostate. Recommended concentration: 10µg/ml.

Antibody detection limit dilution 1:128000.

Approx 110kDa band observed in Human Brain (Hippocampus) lysates (calculated MW of 110kDa according to NP_000213.1). An additional band of unknown identity was also consistently observed at 35kDa. This band was successfully blocked by incubation with the immunizing peptide. Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, IF, IHC, FC