Goat Anti-LHCGR Antibody

$423.00

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SKU: EB07460
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
CG receptor|LH receptor|lutropin/choriogonadotropin receptor|luteinizing hormone receptor|hLHR|LHR|LGR2|LCGR|luteinizing hormone/choriogonadotropin receptor|LHCGR
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the plasma membranes of HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

Accession ID
NP_000224.2
Blocking Peptide
EBP07460
Immunogen
Peptide with sequence CQGTALLDKTRYTE, from the C Terminus of the protein sequence according to NP_000224.2.
Peptide Sequence
CQGTALLDKTRYTE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
3973
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary experiments gave an approx 35kDa band in K562 cell lysates after 0.1µg/ml antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please note that we cannot currently find an explanation in the literature for this band, given the calculated size of 78.6kDa according to NP_000224.2.
Application Type
Pep-ELISA, IF, FC