Goat Anti-LIMS2 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB10398
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
FLJ10044|ILK-binding protein|LIM and senescent cell antigen-like domains 2|OTTHUMP00000203848|OTTHUMP00000203850|OTTHUMP00000203866|PINCH2|LIMS2
Accession ID
NP_001129509.2; NP_001154875.1
Blocking Peptide
EBP10398
Immunogen
Peptide with sequence C-RWSNMSDALA, from the internal region of the protein sequence according to NP_001129509.2; NP_001154875.1.
Product Comments
This antibody is expected to recognize isoform 1 (NP_001129509.2) and isoformn 3 (NP_001154875.1).
Peptide Sequence
C-RWSNMSDALA
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
55679
Product Grade
IHC Results
Paraffin embedded Human Spleen. Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Preliminary experiments gave an approx 80kDa band in Human Heart and Mouse Heart lysates after 0.5 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.2kDa according to NP_001129509.2. The 80kDa band was successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA, IHC

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