Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names malate dehydrogenase, mitochondrial|MOR1|MGC:3559|MDH|M-MDH|malate dehydrogenase 2, NAD (mitochondrial)|MDH2 | |
Accession ID NP_005909.2 | |
Blocking Peptide EBP13027 | |
Immunogen Peptide with sequence C-TRDDLFNTNAT, from the internal region of the protein sequence according to NP_005909.2. | |
Peptide Sequence C-TRDDLFNTNAT | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Pig, Cow | |
Reactive Species Human, Mouse, Rat, Pig | |
Human Gene ID 4191 | |
Mouse Gene ID 17448 | |
Rat Gene ID 81829 | |
Product Grade  | |
IHC Results Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:8000. | |
Western Blot Approx 35kDa band observed in lysates of cell lines HeLa, HepG2 and NIH3T3 (calculated MW of 35.5kDa according to Human NP_005909.2 and 35.6kDa according to Mouse NP_032643.2 ). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. Approx 35kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 35.5kDa according to Human NP_005909.2 and 35.6kDa according to Mouse NP_032643.2, Rat NP_112413.2 and Pig NP_001231082.1 ). Recommended concentration: 0.01-0.03µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human, PMID: 38609156. | |
Application Type Pep-ELISA, WB, IHC |
Goat Anti-MDH2 Antibody
$423.00
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Selected References [{"pmid": 38609156, "intro": "This antibody has been successfully used in WB on Human:", "title": "Cytosolic RNA binding of the mitochondrial TCA cycle enzyme malate dehydrogenase.", "author": "Michelle Noble, Aindrila Chatterjee, Thileepan Sekaran, Thomas Schwarzl, Matthias W Hentze", "journal": "RNA. 2024 Jun 17;30(7):839-853."}] |