Goat Anti-MK2 / MAPKAPK2 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB11929
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
MAPKAP kinase 2|MAPK-activated protein kinase 2|MAP kinase-activated protein kinase 2|MK2|MK-2|MAPKAP-K2|mitogen-activated protein kinase-activated protein kinase 2|MAPKAPK2
Usage Summary
Immunoprecipitation: MK2/MK3 double knockout MEFs retrovirally transduced wiith MK2 or empty vector were lysed from confluent plates and used for IP with 1.5ug EB11929 or EB11930. Western blots of the IP were labelled with rabbit anti-MK2 (CST). An approx 45kDa of MK2 is only precipitated from lysates of those KO MEFs that have been rescued by the MK2 expression construct as described in PMID: 19743408. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany.
Accession ID
NP_004750.1; NP_116584.2
Blocking Peptide
EBP11929
Immunogen
Peptide with sequence C-SRVLKEDKERWED, from the internal region (near C Terminus) of the protein sequence according to NP_004750.1; NP_116584.2.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_004750.1; NP_116584.2).
Peptide Sequence
C-SRVLKEDKERWED
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human, Mouse
Human Gene ID
9261
Mouse Gene ID
17164
Rat Gene ID
289014
Product Grade
IHC Results
Paraffin embedded Human Heart. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 60kDa band observed in Mouse Embryonic Fibroblasts (MEF) lysates, not present in the knockout MEFs and reappearing as a 45kDa band upon reintroduction of MK2 gene expression through viral transduction (calculated MW of 44.0kDa according to Mouse NP_032577.1). The observed molecular weights correspond to earlier findings in literature with different antibodies (Menon et al, Cell Motil Cytoskeleton. 2009 Dec;66(12):1041-7. PMID: 19743408). There is no cross-reactivity to MK5. Data obtained from Dr. M. B. Menon, Inst. Biochemistry, Hannover Medical School, Germany. Recommended concentration: 0.5-2µg/ml.
Application Type
Pep-ELISA, WB, IP, IHC

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