Goat Anti-MNSOD (aa119-130) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

superoxide dismutase [Mn], mitochondrial| manganese-containing superoxide dismutase| indophenoloxidase B| Mn superoxide dismutase| MVCD6| MNSOD| IPOB| RP1-56L9.2| superoxide dismutase 2, mitochondrial|SOD2

NP_000627.2; NP_001019637.1

EBP10972

Peptide with sequence C-EAIKRDFGSFDK, from the internal region of the protein sequence according to NP_000627.2; NP_001019637.1.

NB: The immunizing peptide represents the acetylation site including K122 according to isoform A. This antibody is expected to recognize both reported isoforms (NP_000627.2; NP_001019637.1) . Reported variants represent identical protein: NP_000627.2; NP_001019637.1.

C-EAIKRDFGSFDK

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Pig, Cow, Zebrafish

Human, Mouse, Rat, Pig

6648

20656

24787

Paraffin embedded Human Skeletal Muscle. Recommended concentration: 5µg/ml.

Antibody detection limit dilution 1:64000.

Approx 24kDa band observed in Human Brain (cerebellum) lysates and in Mouse and Rat Brain and Spinal Cord lysates, while approx 26kDa band was observed in lysates of cell lines HeLa, HepG2, HEK293 and NIH3T3 (calculated MW of 24.8kDa according to Human NP_000627.2, and 24.6kDa according to Mouse NP_038699.2). Recommended concentration: 0.01-0.1µg/ml. Primary incubation was 1 hour.

Pep-ELISA, WB, IHC