Goat Anti-MTHFR Antibody

$423.00

Category: Primary Antibodies
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SKU: EB09354
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
MTHFR|5, 10-methylenetetrahydrofolate reductase (NADPH)|OTTHUMP00000002367|OTTHUMP00000002368|methylenetetrahydrofolate reductase intermediate form
Accession ID
NP_005948.3
Blocking Peptide
EBP09354
Immunogen
Peptide with sequence C-NRPTQNARETEAP, from the C Terminus of the protein sequence according to NP_005948.3.
Peptide Sequence
C-NRPTQNARETEAP
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
4524
Product Grade
IHC Results
Paraffin embedded Human Spleen. Recommended concentration: 3.75µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Preliminary experiments gave an approx 28kDa band in Human Brain (Cerebellum), Skeletal Muscle and Placenta lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 74.6kDa according to NP_005948.3. The 28kDa band was successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA, IHC

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