Goat Anti-MTNR1B Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

MTNR1B|melatonin receptor 1B|MEL-1B-R|MT2|melatonin receptor MEL1B|melatonin receptor type 1B

NP_005950.1

EBP09089

Peptide with sequence C-QDASKGSHAEGLQSP, from the C Terminus of the protein sequence according to NP_005950.1.

C-QDASKGSHAEGLQSP

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

Human

4544

In paraffin embedded Human Cerebellum shows membranous staining primarily in Purkinje cells. Recommended concentration, 3-6µg/ml.

Antibody detection limit dilution 1:32000.

Preliminary experiments gave approx .48kDa and 37kDa bands in Human Brain (Cerebellum) lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.2kDa according to NP_005950.1. The 48kDa and 37kDa bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA, IHC