Goat Anti-NCKAP1 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB11695
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
p125Nap1|OTTHUMP00000163461|OTTHUMP00000163460|NCK-associated protein 1|NCKAP1|NAP125|NAP1|NAP 1|MGC8981|membrane-associated protein HEM-2|KIAA0587|HEM2|FLJ11291
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of U2OS and A431 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_038464.1; NP_995314.1
Blocking Peptide
EBP11695
Immunogen
Peptide with sequence C-TPREYLTSHLEIR, from the internal region of the protein sequence according to NP_038464.1; NP_995314.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_038464.1; NP_995314.1).
Peptide Sequence
C-TPREYLTSHLEIR
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human
Human Gene ID
10787
Mouse Gene ID
50884
Rat Gene ID
58823
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Preliminary experiments gave an approx 100kDa band in Human Cerebellum, Cerebral Cortex, Skin and Tonsil lysates after 0.5µg/ml antibody staining, and was successfully blocked by incubation with the immunizing peptide. Please note the calculated MWt.is 129kDa according to NP_038464.1 and approx. 110-125kDa bands have been observed by other sources.
Application Type
Pep-ELISA, IF, FC

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