Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names NDUFS8|TYKY|NADH-ubiquinone oxidoreductase 23 kDa subunit|NADH dehydrogenase ubiquinone Fe-S 8|NADH dehydrogenase (ubiquinone) Fe-S protein 8, 23kDa (NADH-coenzyme Q reductase)|complex I-23kD|CI23KD | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_002487.1 | |
Blocking Peptide EBP10193 | |
Immunogen Peptide with sequence C-EAEIAANIQAD, from the internal region of the protein sequence according to NP_002487.1. | |
Peptide Sequence C-EAEIAANIQAD | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Cow | |
Reactive Species Human, Rat | |
Human Gene ID 4728 | |
Mouse Gene ID 225887 | |
Rat Gene ID 293652 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:4000. | |
Western Blot Approx. 25kDa band observed in Rat Heart and Brain lysates and in preliminary testing of Rat Kidney, Human Heart lysate and A549 cell lysate (calculated MW of 24kDa according to Rat : NP_001099792.1). Recommended concentration: 0.5-2µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IF, FC |
Goat Anti-NDUFS8 Antibody
$464.00
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