Goat Anti-NEFM / NF-M Antibody

$423.00

Category: Primary Antibodies
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SKU: EB09641
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
neurofilament-3 (150 kD medium)|neurofilament, medium polypeptide 150kDa|neurofilament triplet M protein|NFM|NF-M|NEF3|neurofilament, medium polypeptide|NEFM
Accession ID
NP_005373.2
Blocking Peptide
EBP09641
Immunogen
Peptide with sequence C-QASHITVERKDYLK, from the internal region of the protein sequence according to NP_005373.2.
Product Comments
This antibody is expected to recognize reported isoform 1 (NP_005373.2) only.
Peptide Sequence
C-QASHITVERKDYLK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human, Mouse
Human Gene ID
4741
Mouse Gene ID
18040
Rat Gene ID
24588
Product Grade
IHC Results
Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 170-200kDa band observed in Mouse Brain lysates (calculated MW of 102kDa according to NP_005373.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC

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