Goat Anti-NELL2 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB12278
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
protein kinase C-binding protein NELL2|neural epidermal growth factor-like 2|NEL-related protein 2|NEL-like protein 2|NRP2|NEL-like 2 (chicken)|NELL2
Accession ID
NP_001138579.1; NP_006150.1; NP_001138581.1; NP_001138582.1
Blocking Peptide
EBP12278
Immunogen
Peptide with sequence DPCQADTIRNDITKT, from the internal region (near C terminus) of the protein sequence according to NP_001138579.1; NP_006150.1; NP_001138581.1; NP_001138582.1.
Product Comments
This antibody is expected to recognize all reported isoforms (NP_001138579.1; NP_006150.1; NP_001138581.1; NP_001138582.1). Reported variants represent identical protein: NP_001138580.1, NP_006150.1.
Peptide Sequence
DPCQADTIRNDITKT
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human
Human Gene ID
4753
Mouse Gene ID
54003
Rat Gene ID
81734
Product Grade
IHC Results
Paraffin embedded Human Prostate. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Preliminary experiments gave an approx 80kDa band in Human Brain (cortex and hippocampus) lysates after 0.5µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 94.7kDa for the mature form (aa22-866) according to NP_001138579.1 and 88.8kDa (aa22-816) according to NP_001138580.1. The 80kDa band was successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA, IHC

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