Goat Anti-NOVA1 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB08387
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
ventral neuron-specific protein 1|paraneoplastic Ri antigen|Nova-1|neuro-oncological ventral antigen 1|NOVA1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of U2OS cells. Recommended concentration: 10ug/ml.

Accession ID
NP_002506.2 ; NP_006480.2 ; NP_006482.1; NP_001353321.1; NP_001353322.1; NP_001353323.1
Blocking Peptide
EBP08387
Immunogen
Peptide with sequence C-REMPQNVAKTEPVS, from the internal region of the protein sequence according to NP_002506.2 ; NP_006480.2 ; NP_006482.1; NP_001353321.1; NP_001353322.1; NP_001353323.1.
Product Comments
This antibody is expected to recognise all reported isoforms listed.
Peptide Sequence
C-REMPQNVAKTEPVS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Cow
Reactive Species
Human
Human Gene ID
4857
Product Grade
IHC Results
Paraffin embedded Human Brain (Cortex) and Heart. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx. 50-55kDa band observed in Human Breast Cancer lysates, and in preliminary testing of Human Kidney lysate (calculated MW of 51.7kDa according to NP_002506.2). Recommended concentration: 0.01-0.03µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, IF, FC

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