Goat Anti-Nucleophosmin / NPM1 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB06684
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
nucleophosmin 1|MGC104254|nucleophosmin/nucleoplasmin family, member 1|nucleolar phosphoprotein B23|numatrin|nucleophosmin (nucleolar phosphoprotein B23, numatrin)|B23|NPM|NPM1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nucleoli of HeLa cells. Recommended concentration: 10µg/ml.
Accession ID
NP_001341935.1; NP_954654.1; NP_001341936.1; NP_001341939.1
Blocking Peptide
EBP06684
Immunogen
Peptide with sequence C-QEAIQDLWQWRKSL, from the C Terminus of the protein sequence according to NP_001341935.1; NP_954654.1; NP_001341936.1; NP_001341939.1.
Peptide Sequence
C-QEAIQDLWQWRKSL
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human, Mouse
Human Gene ID
4869
Product Grade
IHC Results
Paraffin embedded Human Testis. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Approx 37kDa band observed in lysates of cell lines Daudi, Jurkat, K562 and NIH3T3 and in Mouse Spleen lysates (calculated MW of 32.6kDa according to Human NP_001341935.1 and Mouse NP_032748.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.01-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, IHC

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