Goat Anti-ORP9 / OSBPL9 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB06158
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
OSBPL9|ORP9|FLJ12492|FLJ14629|FLJ14801|FLJ32055|FLJ34384|MGC15035|oxysterol binding protein-like 9|OSBP-related protein 9|oxysterol-binding protein-related protein 9
Accession ID
NP_078862.4; NP_683702.1; NP_683704.2; NP_683705.1; NP_683706.3; NP_683707.3
Blocking Peptide
EBP06158
Immunogen
Peptide with sequence C-EPLLKRLGAAKH, from the C Terminus of the protein sequence according to NP_078862.4; NP_683702.1; NP_683704.2; NP_683705.1; NP_683706.3; NP_683707.3.
Product Comments
This antibody is expected to recognise all six human isoforms (NP_078862.4; NP_683702.1; NP_683704.2; NP_683705.1; NP_683706.3; NP_683707.3). Reported variants represent identical protein (NP_683702.1; NP_683703.1).
Peptide Sequence
C-EPLLKRLGAAKH
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human
Human Gene ID
114883
Product Grade
IHC Results
In paraffin embedded Human Lung shows staining of alveolar macrophages. . Recommended concentration, 2-4µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary experiments gave bands at approx 150kDa and 19kDa in lysates of Human Brain, and Testis and 55kDa in lysates of Human Placenta and Testes after 0.5 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the different calculated sizes of the six reported isoforms (a: 62.5kDa for NP_683702.1 and NP_683703.1; b: 63.9kDa for NP_683704.1; c: 70.2kDa for NP_683705.1; d: 81.8kDa for NP_683706.1; e: 83.2 kDa for NP_078862.2; f: 84.3kDa for NP_683707.1). All detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA, IHC

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