Goat Anti-OTUD4 Antibody

$423.00

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SKU: EB09121
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
OTU domain containing 4 protein|HIV-1 induced protein HIN-1|KIAA1046|HSHIN1|HIN1|DUBA6|DKFZp434I0721|OTU domain containing 4|OTUD4
Usage Summary
Immunofluorescence: Strong expression of the protein seen in A413 and U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_955356.1; NP_001096123.1; NP_001352986.1
Blocking Peptide
EBP09121
Immunogen
Peptide with sequence C-SRSRDEGYQYHRNVR , from the C Terminus (near) of the protein sequence according to NP_955356.1; NP_001096123.1; NP_001352986.1.
Product Comments
This antibody is expected to recognize reported isoforms 1 (NP_955356.1), 3 (NP_001096123.1), and 4 (NP_001352986.1).
Peptide Sequence
C-SRSRDEGYQYHRNVR
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
54726
Mouse Gene ID
73945
Rat Gene ID
307774
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Preliminary testing showed a band at approx. 110kDa in Human Skeletal Muscle lysate after 2ug/ml antibody staining. (calculated MW of 117kDa according to NP_955356.1). This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IF, FC