Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names Rho/Rac guanine nucleotide exchange factor 18|P114-RhoGEF|Rho-specific guanine nucleotide exchange factor p114|MGC15913|KIAA0521|P114-RHO-GEF|Rho/Rac guanine nucleotide exchange factor (GEF) 18|ARHGEF18 | |
Usage Summary Immunofluorescence: This antibody has been successfully used in IF on Human, PMID: 36404918. | |
Accession ID NP_056133.2; NP_001124427.1 | |
Blocking Peptide EBP06163 | |
Immunogen Peptide with sequence C-SAKEDASKEDVIFF, from the C Terminus of the protein sequence according to NP_056133.2; NP_001124427.1. | |
Product Comments This antibody is expected to recognize both reported isoforms (NP_056133.2; NP_001124427.1). | |
Peptide Sequence C-SAKEDASKEDVIFF | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human | |
Human Gene ID 23370 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Approx 140+90kDa band observed in lysates of cell line Daudi and Caco-2 (calculated MW of 114kDa according to NP_056133.2 and 131kDa accoeding to NP_001124427.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human, PMID: 36404918. | |
Application Type Pep-ELISA, WB, IF |
Goat Anti-P114-RHO-GEF / ARHGEF18 Antibody
$423.00
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Selected References [{"pmid": 36404918, "intro": "This antibody has been successfully used in WB and IF on Human:", "title": "Downregulation of protein kinase C gamma reduces epithelial property and enhances malignant phenotypes in colorectal cancer cells.", "author": "Reiko Satow, Yudai Suzuki, Shinobu Asada, Sae Ota, Masashi Idogawa, Shiori Kubota, Noi Ikeo, Atsuko Yoneda, Kiyoko Fukami", "journal": "iScience. 2022 Nov 4;25(12):105501."}, {"pmid": 23698346, "intro": "This antibody (previous batch) has been successfully used in WB on Medaka:", "title": "ArhGEF18 regulates RhoA-Rock2 signaling to maintain neuro-epithelial apico-basal polarity and proliferation.", "author": "Herder C, Swiercz JM, Müller C, Peravali R, Quiring R, Offermanns S, Wittbrodt J, Loosli F.", "journal": "Development. 2013 Jul;140(13):2787-97."}, {"pmid": 23185572, "intro": "This antibody (previous batch) has been successfully used in WB on Human:", "title": "Stimulation of Cortical Myosin Phosphorylation by p114RhoGEF Drives Cell Migration and Tumor Cell Invasion.", "author": "Terry SJ, Elbediwy A, Zihni C, Harris AR, Bailly M, Charras GT, Balda MS, Matter K.", "journal": "PLoS One. 2012;7(11):e50188."}, {"pmid": 21258369, "intro": "This antibody (previous batch) has been successfully used in WB, IF and IP on Human:", "title": "Spatially restricted activation of RhoA signalling at epithelial junctions by p114RhoGEF drives junction formation and morphogenesis.", "author": "Terry SJ, Zihni C, Elbediwy A, Vitiello E, Leefa Chong San IV, Balda MS, Matter K.", "journal": "Nat Cell Biol. 2011 Feb;13(2):159-66."}] |