Goat Anti-PALS / MPP5 Antibody

$464.00

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SKU: EB06223
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
stardust|membrane protein, palmitoylated 5|MAGUK p55 subfamily member 5|stardust (Drosophila)|membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5)|FLJ12615|PALS1|MPP5
Usage Summary
Immunofluorescence: Strong expression of the protein seen in U2OS and HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

Accession ID
NP_071919.2; NP_001243479.1
Blocking Peptide
EBP06223
Immunogen
Peptide with sequence NKASPPFPLISNAQ-C, from the N Terminus of the protein sequence according to NP_071919.2; NP_001243479.1.
Peptide Sequence
NKASPPFPLISNAQ-C
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Cow, Dog, Pig
Reactive Species
Human
Human Gene ID
64398
Product Grade
IHC Results
Paraffin embedded Human Small Intestine and Kidney. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Preliminary testing showed a band at approx 75kDa in Human Cerebellum lysate after 1µg/ml antibody staining (calculated MW of 73.4kDa according to NP_001243479.1). Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, IF, IHC, FC