Goat Anti-PAR6alpha / PARD6A Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

partitioning-defective protein 6|partitioning defective-6 homolog alpha|par-6 partitioning defective 6 homolog alpha|Tax-interacting protein 40|PAR6C|PAR6|par-6 (partitioning defective 6, C.elegans) homolog alpha|Tax interaction protein 40|par-6 partitioning defective 6 homolog alpha (C.elegans)|TIP-40|PAR-6A|TAX40|PAR-6|PAR6alpha|PARD6A

Flow Cytometry: Flow cytometric analysis of Jurkat cells. Recommended concentration: 10ug/ml.

NP_058644.1; NP_001032358.1

EBP06228

Peptide with sequence C-GSRIRGDGSGFSL, from the C Terminus of the protein sequence according to NP_058644.1; NP_001032358.1.

This antibody is expected to recognise both reported isoforms (NP_058644.1; NP_001032358.1).

C-GSRIRGDGSGFSL

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human

Human

50855

In paraffin embedded Human Pancreas, shows strong cytoplasmic staining in rare cells of intralobular ducts. Recommended concentration: 10µg/ml. Also tested in paraffin embedded Human Heart where it shows speckled staining on myocardial fibres in transverse section. Recommended concentration: 5-10µg/ml.

Antibody detection limit dilution 1:8000.

Approx 40kDa band observed in lysates of cell lines Jurkat and glioblastoma U251 (calculated MW of 37.4kDa according to NP_058644.1) . Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, IHC, FC