Goat Anti-PAX6 (internal) Antibody

$423.00

Category: Primary Antibodies
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SKU: EB07772
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
paired box homeotic gene-6|paired box homeotic gene 6 (aniridia, keratitis)|paired box gene 6|WAGR|MGDA|MGC17209|D11S812E|AN2|AN|paired box gene 6 (aniridia, keratitis)|PAX6
Usage Summary
Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended concentration: 10ug/ml.
Accession ID
NP_000271.1; NP_001595.2; NP_001297088.1; NP_001355839.1; NP_001355840.1; NP_001355841.1; NP_001355844.1; NP_001355847.1; NP_001355849.1; NP_001355850.1; NP_001355851.1; NP_001355857.1; NP_001355858.1; NP_001355859.1
Blocking Peptide
EBP07772
Immunogen
Peptide with sequence C-REEKLRNQRRQASN, from the internal region of the protein sequence according to NP_000271.1; NP_001595.2; NP_001297088.1; NP_001355839.1; NP_001355840.1; NP_001355841.1; NP_001355844.1; NP_001355847.1; NP_001355849.1; NP_001355850.1; NP_001355851.1; NP_001355857.1; NP_001355858.1; NP_001355859.1.
Product Comments
This antibody is expected to recognize all reported isoforms: NP_000271.1; NP_001595.2; NP_001297088.1; NP_001355839.1; NP_001355840.1; NP_001355841.1; NP_001355844.1; NP_001355847.1; NP_001355849.1; NP_001355850.1; NP_001355851.1; NP_001355857.1; NP_001355858.1; NP_001355859.1.
Peptide Sequence
C-REEKLRNQRRQASN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human, Mouse
Human Gene ID
5080
Mouse Gene ID
18508
Rat Gene ID
25509
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 26kDa band observed in lysates of cell lines U251 and RAW264.7 and in nuclear lysates of cell lines K562 and HeLa, all successfully blocked by incubation with the immunising peptide (calculated MW of 24.8kDa according to Human NP_001355859.1 and 31.8 according to Mouse NP_001297075.1). Recommended concentration: 0.03-1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, FC

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