Goat Anti-PGC1A/PPARGC1A Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

peroxisome proliferative activated receptor, gamma, coactivator 1, alpha|gamma, coactivator 1|peroxisome proliferative activated receptor|ligand effect modulator-6|PPAR gamma coactivator-1|PPAR gamma coactivator variant form|PPARGC1|PGC1|PGC-1v|PGC-1(alpha)|LEM6|peroxisome proliferator-activated receptor gamma, coactivator 1 alpha|PPARGC1A|PGC1A

Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of U2OS and HeLa cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml.

NP_037393.1; NP_001317680.1; NP_001317681.1; NP_001317682.1; NP_001341756.1;

EBP07856

Peptide with sequence C-DGLFDDSEDESDK, from the internal region of the protein sequence according to NP_037393.1; NP_001317680.1; NP_001317681.1; NP_001317682.1; NP_001341756.1;.

C-DGLFDDSEDESDK

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Cow

Human

10891

19017

83516

Paraffin embedded Human Kidney. Recommended concentration: 6-8µg/ml.

Antibody detection limit dilution 1:2000.

Preliminary testing showed a band at approx 90kDa in nuclear lysates of cell lines A431 and HEK293 after 0.3-1µg/ml antibody staining (calculated MW of 91.0kDa according to Human NP_037393.1). Primary incubation 1 hour at room temperature.

Pep-ELISA, IF, IHC, FC