Goat Anti-POU3F3 / BRN1 / OCT8 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB10027
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
octamer-binding transcription factor 8|octamer-binding protein 8|oct-8|brn-1|brain-specific homeobox/POU domain protein 1|OTF-8|POU3F3|POU domain, class 3, transcription factor 3|POU class 3 homeobox 3|OTF8|BRN1|Brain-1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of Neuro-2a cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of Neuro-2a cells. Recommended concentration: 10ug/ml.

Accession ID
NP_006227.1
Blocking Peptide
EBP10027
Immunogen
Peptide with sequence C-HMLSHAHQWVTAL, from the internal region of the protein sequence according to NP_006227.1.
Peptide Sequence
C-HMLSHAHQWVTAL
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat
Reactive Species
Mouse
Human Gene ID
5455
Mouse Gene ID
18993
Rat Gene ID
192109
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:4000.
Western Blot
Approx 48kDa band observed in Mouse Spinal Cord lysates and approx. 50kDa in Mouse Brain lysates (calculated MW of 50.3kDa according to Human NP_006227.1 and 50.2kDa according to Mouse NP_032926.2). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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