Goat Anti-PPP1R15A / GADD34 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB05034
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
protein phosphatase 1, regulatory subunit 15A|growth arrest and DNA-damage-inducible 34|protein phosphatase 1, regulatory (inhibitor) subunit 15A|GADD34|PPP1R15A
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa and HepG2 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_055145.3
Blocking Peptide
EBP05034
Immunogen
Peptide with sequence C-AAALDLSGRRG, from the C Terminus of the protein sequence according to NP_055145.3.
Peptide Sequence
C-AAALDLSGRRG
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
23645
Product Grade
IHC Results
In paraffin embedded Human Liver shows cytoplasmic staining consistent with ER in the hepatocytes. Recommended concentration: 2-4µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 75kDa band observed in lysates of cell line HepG2 (calculated MW of 73.4kDa according to NP_055145.2). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. Negative Control: KLY (Kelly Human neuroblastoma) cell lysate.
Application Type
Pep-ELISA, WB, IHC, IF, FC

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